Western Blot:Article Title: Cargo receptor Surf4 regulates endoplasmic reticulum export of proinsulin in pancreatic β-cells
Article Snippet: .. The following antibodies were purchased: mouse monoclonal anti-pan-actin (C4; Millipore, Tokyo, Japan, 1:3000 for immunoblotting), mouse monoclonal anti-GFP (3E6; Thermo Fisher Scientific, Tokyo, Japan, 1:1000 for immunoblotting), goat polyclonal anti-GFP conjugated to HRP (Fitzgerald Industries International, Flanders, NJ, USA, 1:1000 for immunoblotting), goat polyclonal anti-Surf4 (S-12; Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:1000 for immunoblotting), rabbit monoclonal anti-PDI (C81H6; Cell Signaling Technology, Danvers, MA, USA, 1:100 for immunostaining), rabbit polyclonal anti-ERGIC-53/p58 (E1031; Sigma-Aldrich, Tokyo, Japan, 1:500 for immunostaining), rabbit anti-rat chromogranin A (Y291; Yanaihara Institute Inc., Fujinomiya, Shizuoka, Japan, 1:1000 for immunostaining), mouse monoclonal anti-insulin (L6B10; Cell Signaling Technology, Danvers, MA, USA, 1:500 for immunoblotting), mouse monoclonal anti-proinsulin (3A1; ab8301, Abcam, Cambridge, MA, USA, 1:300 for immunostaining), normal mouse IgG (12-371; Merck Millipore, Tokyo, Japan), mouse monoclonal anti-GM130 (35/GM130; BD Biosciences, San Jose, CA, USA, 1:500 for immunostaining), and mouse monoclonal anti-TGN38 (2/TGN38; BD Biosciences, 1:200 for immunostaining). .. For transient expression of GFP-hHMOX1 or hInsulin-mRFP, cells were transfected with each expression construct using Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s instructions and incubated for 24 h.
Immunostaining:Article Title: Cargo receptor Surf4 regulates endoplasmic reticulum export of proinsulin in pancreatic β-cells
Article Snippet: .. The following antibodies were purchased: mouse monoclonal anti-pan-actin (C4; Millipore, Tokyo, Japan, 1:3000 for immunoblotting), mouse monoclonal anti-GFP (3E6; Thermo Fisher Scientific, Tokyo, Japan, 1:1000 for immunoblotting), goat polyclonal anti-GFP conjugated to HRP (Fitzgerald Industries International, Flanders, NJ, USA, 1:1000 for immunoblotting), goat polyclonal anti-Surf4 (S-12; Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:1000 for immunoblotting), rabbit monoclonal anti-PDI (C81H6; Cell Signaling Technology, Danvers, MA, USA, 1:100 for immunostaining), rabbit polyclonal anti-ERGIC-53/p58 (E1031; Sigma-Aldrich, Tokyo, Japan, 1:500 for immunostaining), rabbit anti-rat chromogranin A (Y291; Yanaihara Institute Inc., Fujinomiya, Shizuoka, Japan, 1:1000 for immunostaining), mouse monoclonal anti-insulin (L6B10; Cell Signaling Technology, Danvers, MA, USA, 1:500 for immunoblotting), mouse monoclonal anti-proinsulin (3A1; ab8301, Abcam, Cambridge, MA, USA, 1:300 for immunostaining), normal mouse IgG (12-371; Merck Millipore, Tokyo, Japan), mouse monoclonal anti-GM130 (35/GM130; BD Biosciences, San Jose, CA, USA, 1:500 for immunostaining), and mouse monoclonal anti-TGN38 (2/TGN38; BD Biosciences, 1:200 for immunostaining). .. For transient expression of GFP-hHMOX1 or hInsulin-mRFP, cells were transfected with each expression construct using Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s instructions and incubated for 24 h.
other:
Article Title: GLP-1 signaling suppresses menin’s transcriptional block by phosphorylation in β cells
Article Snippet: The antibodies and chemicals used in this study were as follows: rabbit polyclonal anti-menin (A300-115A, 1:5,000 dilution; Bethyl), mouse monoclonal anti-Flag (clone M2; F3165, 1:1,000 dilution; Sigma-Aldrich), rabbit polyclonal anti-PKA Cα (4782, 1:1,000 dilution; Cell Signaling Technology), rabbit monoclonal anti-phospho-PKA substrate (RRXS*/T*; 9624, 1:1,000 dilution; Cell Signaling Technology), rabbit monoclonal anti-GFP (2956S, 1:1,000 dilution; Cell Signaling Technology), mouse monoclonal anti-insulin (clone L6B10; 8138, 1:1,000 dilution; Cell Signaling Technology), rabbit monoclonal anti-GAPDH (2118, 1:1,000 dilution; Cell Signaling Technology), rabbit polyclonal anti-Myosin IIa (3403, 1:1,000 dilution; Cell Signaling Technology), rabbit polyclonal anti-histone H3 (trimethyl K4) antibody (ab8580; Abcam), rabbit polyclonal anti-histone H3 (trimethyl K9) antibody (ab8898; Abcam), mouse monoclonal anti-histone H3 (trimethyl K27) antibody (ab6002; Abcam), rabbit polyclonal anti-histone H3 (ab1791; Abcam), mouse monoclonal anti-Suv39H1 (ab12405, 1:1,000 dilution; Abcam), rabbit polyclonal anti-HDAC1 (ab7028, 1:1,000 dilution; Abcam), rabbit polyclonal anti-IgG (ab171870; Abcam), rabbit polyclonal anti-lamin A (ab26300, 1:2,000 dilution; Abcam), rabbit polyclonal anti-XPO6 (ab72333, 1:1,000 dilution; Abcam), rabbit polyclonal anti-acetyl-histone H3 (06–599; Millipore), mouse monoclonal anti-β actin (SC47778, 1:1,000 dilution; Santa Cruz), mouse monoclonal anti-pSer487 menin (1:1,000 dilution; this paper), goat anti-mouse IgG (H+L) HRP conjugate (170–6516, 1:5,000 dilution; Bio-Rad), Goat Anti-Rabbit IgG (H+L) HRP conjugate (170–6515, 1:5,000 dilution; Bio-Rad), Clean-Blot IP detection kit (HRP; 21232, 1:400 dilution; Thermo Scientific), Alexa Fluor 488 goat anti-mouse IgG (H+L; A11001, 1:1,000 dilution; Invitrogen), Ex-4 (1269105; Sigma-Aldrich), forskolin (F6886; Sigma-Aldrich), ATP disodium salt hydrate (FLAAS; Sigma-Aldrich), PKA catalytic subunit from bovine heart (P2645; Sigma-Aldrich), Chaetocin from Chaetomium minutum (C9492; Sigma-Aldrich), trichostatin A (T1952; Sigma-Aldrich), SAHA (SML0061; Sigma-Aldrich), 8-pCPT-2′-O-Me-cAMP (sc-257020; Santa Cruz), dibutyryl-cAMP (sc-201567; Santa Cruz), Rp-cAMPS (sc-24010; Santa Cruz), leptomycin B (9676; Cell Signaling Technology), jasplakinolide (ab141409; Abcam), cytochalasin D (ab143484; Abcam), and latrunculin B (ab144291; Abcam).
Immunocytochemistry:Article Title: Transplantation of insulin-producing cells derived from human mesenchymal stromal/stem cells into diabetic humanized mice
Article Snippet: .. For immunocytochemistry and immunohistochemistry : the primary antibodies used were mouse monoclonal anti-insulin (#8138, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-glucagon (# 8233, Cell Signaling Technology), rabbit polyclonal anti-c-peptide (# 4593, Cell Signaling Technology) and rabbit polyclonal anti-human somatostatin (# GTX39061, Gene Tex, Alton Pkwy Irvine, CA, USA). .. The secondary antibodies used were (H + L Alexa flour 488 conjugate) anti-mouse IgG heavy and light (# 4408, Cell Signaling Technology), and Alexa flour 555 conjugate anti-rabbit IgG (H + L) (# 4413, Cell Signaling Technology).
Article Title: Modulation of naïve mesenchymal stromal cells by extracellular vesicles derived from insulin-producing cells: an in vitro study
Article Snippet: .. Immunocytochemistry The primary antibodies used were mouse monoclonal anti-insulin (Cat #8138, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-glucagon (Cat # 8233, Cell Signaling Technology), rabbit polyclonal anti-c-peptide (Cat # 4593, Cell Signaling Technology), rabbit polyclonal anti-human somatostatin (Cat # GTX39061, Gene Tex, Alton Pkwy Irvine, CA, USA) and rabbit monoclonal anti-GAD65 (Cat # 5843,Cell Signaling Technology). .. The secondary antibodies used were (H + L Alexa Flour 488 conjugate) anti-mouse IgG heavy and light (Cat # 4408, Cell Signaling Technology), and Alexa Flour 555 conjugate anti-rabbit IgG (H + L) (Cat # 4413, Cell Signaling Technology).
Article Title: Modulation of naïve mesenchymal stromal cells by extracellular vesicles derived from insulin-producing cells: an in vitro study.
Article Snippet: .. Immunocytochemistry The primary antibodies used were mouse monoclonal anti-insulin (Cat #8138, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-glucagon (Cat # 8233, Cell Signaling Technology), rabbit polyclonal anti-c-peptide (Cat # 4593, Cell Signaling Technology), rabbit polyclonal anti-human somatostatin (Cat # GTX39061, Gene Tex, Alton Pkwy Irvine, CA, USA) and rabbit monoclonal anti-GAD65 (Cat # 5843,Cell Signaling Technology). .. The secondary antibodies used were (H + L Alexa Flour 488 conjugate) antimouse IgG heavy and light (Cat # 4408, Cell Signaling Technology), and Alexa Flour 555 conjugate anti-rabbit IgG (H + L) (Cat # 4413, Cell Signaling Technology).
Immunohistochemistry:Article Title: Transplantation of insulin-producing cells derived from human mesenchymal stromal/stem cells into diabetic humanized mice
Article Snippet: .. For immunocytochemistry and immunohistochemistry : the primary antibodies used were mouse monoclonal anti-insulin (#8138, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-glucagon (# 8233, Cell Signaling Technology), rabbit polyclonal anti-c-peptide (# 4593, Cell Signaling Technology) and rabbit polyclonal anti-human somatostatin (# GTX39061, Gene Tex, Alton Pkwy Irvine, CA, USA). .. The secondary antibodies used were (H + L Alexa flour 488 conjugate) anti-mouse IgG heavy and light (# 4408, Cell Signaling Technology), and Alexa flour 555 conjugate anti-rabbit IgG (H + L) (# 4413, Cell Signaling Technology).
Incubation:Article Title: Differentiation Potential of Nestin (+) and Nestin (-) Cells Derived from Human Bone Marrow Mesenchymal Stem Cells into Functional Insulin Producing Cells
Article Snippet: .. These included mouse monoclonal anti-nestin (ThermoFisher Scientific, USA), mouse monoclonal anti-insulin, rabbit polyclonal anti-c-peptide (cell Signaling Technology, Danvers, Massachusetts, USA), Thereafter, a washing step using PBS was applied for the cells, followed by an incubation at RT for 2 h using the secondary antibodies (Alexa Fluor 555-conjugated antirabbit IgG (H + L) and Alexa Fluor 488-conjugated antimouse IgG (H + L)) (Cell Signaling Technology, USA). .. The nuclei of these cells were counterstained with DAPI (Invitrogen, UK).
|
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 